?職稱： 教授 | ||||||||||||||||||
系屬： 生物工程與技術(shù)中心 | ?最高學(xué)位： 博士 | |||||||||||||||||
?學(xué)科： 生物化工 | ?所學(xué)專業(yè)： 化學(xué) | |||||||||||||||||
?電子郵箱：fhuang@upc.edu.cn | ||||||||||||||||||
?聯(lián)系電話：0532-86981235 | ||||||||||||||||||
?地址郵編：山東省青島市黃島區(qū)長江西路66號中國石油大學(xué)（華東）化學(xué)工程學(xué)院,266580 | ||||||||||||||||||
?個人主頁：http://pme.esi-integrity.com/ | ||||||||||||||||||
◎學(xué)習(xí)與工作經(jīng)歷
◎研究方向 蛋白質(zhì)工程、酶工程、單分子熒光技術(shù)、熒光成像技術(shù) ◎承擔(dān)科研課題 1）菌視紫紅質(zhì)去折疊態(tài)和折疊中間態(tài)的單分子研究，國家基金面上項目，2011年1月至2013年12月，主持。 2）多孔薄膜和溶液聚集體結(jié)構(gòu)自組裝、理論模擬與性能的基礎(chǔ)研究，國家基金重點項目，2011年1月至2014年12月，參與 3）生物物理與單分子熒光研究，山東省杰出青年基金，2010年10月至2013年10月，主持 4）教育部新世紀(jì)優(yōu)秀人才支持計劃，2011年1月-2013年12月，主持 5）心血管疾病相關(guān)GPCR新藥物靶點的基礎(chǔ)研究，國家重點基礎(chǔ)研究發(fā)展計劃（973計劃），2012年1月至 2016年12月，主持 6）單螺旋跨膜多肽在生物膜中取向的研究，國家自然科學(xué)基金面上項目，2013年1月-2016年12月，主持 7）用于重大惡性腫瘤早期預(yù)警的等離子體納米鍍膜檢測芯片關(guān)鍵技術(shù)研究，2015年7月-2017年6月，主持 8）多態(tài)蛋白狀態(tài)轉(zhuǎn)變的單分子熒光研究，國家自然科學(xué)基金面上項目，2016年01月-2019年12月，主持 ◎獲獎情況 2012年，山東青年五四獎?wù)?/span> 2019年，青島市自然科學(xué)獎三等獎 ◎代表性論文和專利 1Huang, H., Ge, B., Zhang, S., Li, J., Sun, C., Yue, T. & Huang, F. Using Fluorescence Quenching Titration to Determine the Orientation of a Model Transmembrane Protein in Mimic Membranes. Materials2019, 12, 349. 2Huang, H., Ge, B., Sun, C., Zhang, S. & Huang, F. Membrane curvature affects the stability and folding kinetics of bacteriorhodopsin. Process Biochem2019, 76, 111-117. 3Wang, X., Wang, C., Xu, B., Wei, J., Xiao, Y. & Huang, F. Adsorption of intrinsically disordered barnacle adhesive proteins on silica surface. Appl Surf Sci2018, 427, 942-949. 4Wang, X., Chen, H., Lu, X., Chi, H., Li, S. & Huang, F. Probing the interaction mechanisms between transmembrane peptides and the chaperonin GroEL with fluorescence anisotropy. Spectrochim Acta A Mol Biomol Spectrosc2018, 194, 1-7. 5Song, Y., Ge, B., Lao, J., Wang, Z., Yang, B., Wang, X., He, H., Li, J. & Huang, F. Regulation of the Oligomeric Status of CCR3 with Binding Ligands Revealed by Single-Molecule Fluorescence Imaging. Biochemistry2018, 57, 852-860. 6Khan, N. U., He, H., Wang, X., Ge, B., Wang, Q., Liu, X., Lao, J., Wang, Y., Li, J., Wang, Z., Zhou, S. & Huang, F. A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells. Chem Commun2018, 54, 778-781. 7Khan, N. U., Feng, Z., He, H., Wang, Q., Liu, X., Li, S., Shi, X., Wang, X., Ge, B. & Huang, F. A facile plasmonic silver needle for fluorescence-enhanced detection of tumor markers. Anal. Chim. Acta2018, 1040, 120-127. 8Jacob, M. H., D'Souza, R. N., Schwarzlose, T., Wang, X., Huang, F., Haas, E. & Nau, W. M. Method-Unifying View of Loop-Formation Kinetics in Peptide and Protein Folding. J Phys Chem B2018, 122, 4445-4456. 9Huang, H., Yang, B., Ge, B., Lao, J., Zhou, S. & Huang, F. Using self-cleavable ternary fusion pattern for efficient preparation of Bacteriorhodopsin. Process Biochem2018, 64, 206-212. 10He, J., Xi, L., Sun, X., Ge, B., Liu, D., Han, Z., Pu, X. & Huang, F. Enhanced hydrogen production through co-cultivation of Chlamydomonas reinhardtii CC-503 and a facultative autotrophic sulfide-oxidizing bacterium under sulfurated conditions. Int J Hydrogen Energy2018, 43, 15005-15013. 11He, H., Wang, X. & Huang, F. in Membrane Biophysics (eds Hongda Wang & Guohui Li) 147-173 (Springer, 2018). 12He, H., Li, S., Shi, X., Wang, X., Liu, X., Wang, Q., Guo, A., Ge, B., Khan, N. U. & Huang, F. Quantitative Nanoscopy of Small Blinking Graphene Nanocarriers in Drug Delivery. Bioconjug Chem2018, 29, 3658-3666. 13Chi, H., Xu, B., Liu, Z., Wei, J., Li, S., Ren, H., Xu, Y., Lu, X., Wang, X., Wang, X. & Huang, F. Combined thermodynamic and kinetic analysis of GroEL interacting with CXCR4 transmembrane peptides. Biochim. Biophys. Acta2018, 1862, 1576-1583. 14Zhang, H.-T., Zhao, Y.-Y., Ge, B.-S. & Huang, F. Study on The Interaction of Metamorphic Protein Mad2 and Its Ligand Cdc20(121-138). Progress in Biochemistry and Biophysics2017, 44, 232-241. 15Wang, X., Xiao, Y., Wang, C., Niu, J., Ma, J., Wang, H., Li, X., Chen, X. & Huang, F. Hierarchical interfacial structuring of the calcareous base of the barnacle Balanus albicostatus over different length scales. Mater Chem Phys2017, 192, 48-57. 16Wang, X., Wang, Y., He, H., Ma, X., Chen, Q., Zhang, S., Ge, B., Wang, S., Nau, W. M. & Huang, F. Deep-Red Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of the Nucleolar Dynamics in Reverse Transformation of Malignant Cells. ACS Appl Mater Interfaces2017, 9, 17799-17806. 17Wang, X., Wang, C., Pan, M., Wei, J., Jiang, F., Lu, R., Liu, X., Huang, Y. & Huang, F. Chaperonin-Nanocaged Hemin as an Artificial Metalloenzyme for Oxidation Catalysis. ACS Appl Mater Interfaces2017, 9, 25387-25396. 18Lao, J., He, H., Wang, X., Wang, Z., Song, Y., Yang, B., Ullahkhan, N., Ge, B. & Huang, F. Single-Molecule Imaging Demonstrates Ligand Regulation of the Oligomeric Status of CXCR4 in Living Cells. J Phys Chem B2017, 121, 1466-1474. 19He, H., Liu, X., Li, S., Wang, X., Wang, Q., Li, J., Wang, J., Ren, H., Ge, B., Wang, S., Zhang, X. & Huang, F. High-Density Super-Resolution Localization Imaging with Blinking Carbon Dots. Anal. Chem.2017, 89, 11831-11838. 20Ge, B., Lao, J., Li, J., Chen, Y., Song, Y. & Huang, F. Single-molecule imaging reveals dimerization/oligomerization of CXCR4 on plasma membrane closely related to its function. Sci Rep2017, 7, 16873. 21Zhao, Y., Li, L., Wu, C., Jiang, X., Ge, B., Ren, H. & Huang, F. Stable folding intermediates prevent fast interconversion between the closed and open states of Mad2 through its denatured state. Protein Eng Des Sel2016, 29, 23-29. 22Wang, X., Wang, Y., He, H., Chen, X., Sun, X., Sun, Y., Zhou, G., Xu, H. & Huang, F. Steering graphene quantum dots in living cells: lighting up the nucleolus. J Mater Chem B2016, 4, 779-784. 23Wang, X., He, H., Wang, Y., Wang, J., Sun, X., Xu, H., Nau, W. M., Zhang, X. & Huang, F. Active tumor-targeting luminescent gold clusters with efficient urinary excretion. Chem Commun2016, 52, 9232-9235. 24He, H., Wang, Z., Cheng, T., Liu, X., Wang, X., Wang, J., Ren, H., Sun, Y., Song, Y., Yang, J., Xia, Y., Wang, S., Zhang, X. & Huang, F. Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor Imaging. ACS Appl Mater Interfaces2016, 8, 28529-28537. 25He, H., Wang, X., Cheng, T., Xia, Y., Lao, J., Ge, B., Ren, H., Khan, N. U. & Huang, F. An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chem--Eur J2016, 22, 5863-5867. 26Chi, H., Wang, X., Li, J., Ren, H. & Huang, F. Chaperonin-enhanced Escherichia coli cell-free expression of functional CXCR4. J Biotechnol2016, 231, 193-200. 27Yu, D., Wang, M., Zhu, G., Ge, B., Liu, S. & Huang, F. Enhanced photocurrent production by bio-dyes of photosynthetic macromolecules on designed TiO2 film. Sci Rep2015, 5, 9375. 28Wang, X., Sun, X., He, H., Yang, H., Lao, J., Song, Y., Xia, Y., Xu, H., Zhang, X. & Huang, F. A two-component active targeting theranostic agent based on graphene quantum dots. J. Mater. Chem. B2015, 3, 3583--3590. 29Wang, X., Huang, H., Sun, C. & Huang, F. Structural analysis of bacteriorhodopsin solubilized by lipid-like phosphocholine biosurfactants with varying micelle concentrations. J Colloid Interface Sci2015, 437, 170-180. 30Ge, B., Wang, M., Li, J., Liu, J. & Huang, F. Maltose binding protein facilitates functional production of engineered human chemokine receptor 3 in Escherichia coli. Process Biochem2015, 50, 285-293. 31Chi, H., Wang, X., Li, J., Ren, H. & Huang, F. Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES. Sci Rep2015, 5, 17037. 32Wang, X., Sun, X., Lao, J., He, H., Cheng, T., Wang, M., Wang, S. & Huang, F. Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery. Colloids Surf. B2014, 122, 638-644. 33Song, B., Sun, Q., Li, H., Ge, B., Pan, J. S., Wee, A. T. S., Zhang, Y., Huang, S., Zhou, R., Gao, X., Huang, F. & Fang, H. Irreversible Denaturation of Proteins through Aluminum-Induced Formation of Backbone Ring Structures. Angew Chem, Int Ed2014, 53, 6358-6363. 34Huang, F., Johnson, C. M., Petrovich, M. & Fersht, A. R. Don't waste good methods on bad buffers and ambiguous data. Proc Natl Acad Sci U S A2013, 110, E331-332. 35Leith, J. S., Tafvizi, A., Huang, F., Uspal, W. E., Doyle, P. S., Fersht, A. R., Mirny, L. A. & van Oijen, A. M. Sequence-dependent sliding kinetics of p53. Proc Natl Acad Sci U S A2012, 109, 16552-16557. 36Tafvizi, A., Huang, F., Fersht, A. R., Mirny, L. A. & van Oijen, A. M. A single-molecule characterization of p53 search on DNA. Proc Natl Acad Sci U S A2011, 108, 563-568. 37Rajagopalan, S., Huang, F. & Fersht, A. R. Single-Molecule characterization of oligomerization kinetics and equilibria of the tumor suppressor p53. Nucleic Acids Res.2011, 39, 2294-2303. 38Huang, F., Ying, L. & Fersht, A. R. Direct observation of barrier-limited folding of BBL by single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci USA2009, 106, 16239-16244. 39Huang, F., Rajagopalan, S., Settanni, G., Marsh, R. J., Armoogum, D. A., Nicolaou, N., Bain, A. J., Lerner, E., Haas, E., Ying, L. & Fersht, A. R. Multiple conformations of full-length p53 detected with single-molecule fluorescence resonance energy transfer. Proc Natl Acad Sci USA2009, 106, 20758-20763. 40Huang, F., Lerner, E., Sato, S., Amir, D., Haas, E. & Fersht, A. R. Time-resolved fluorescence resonance energy transfer study shows a compact denatured state of the B domain of protein A. Biochemistry2009, 48, 3468-3476. 41Tafvizi, A., Huang, F., Leith, J. S., Fersht, A. R., Mirny, L. A. & van Oijen, A. M. Tumor suppressor p53 slides on DNA with low friction and high stability. Biophys. J.2008, 95, L01-L03. 42Huang, F., Sato, S., Sharpe, T. D., Ying, L. & Fersht, A. R. Distinguishing between cooperative and unimodal downhill protein folding. Proc Natl Acad Sci USA2007, 104, 123-127. 43Huang, F., Hudgins, R. R. & Nau, W. M. Primary and secondary structure dependence of peptide flexibility assessed by fluorescence-based measurement of end-to-end collision rates. J Am Chem Soc2004, 126, 16665-16675. 44Huang, F. & Nau, W. M. A conformational flexibility scale for amino acids in peptides. Angew Chem Int Ed2003, 42, 2269-2272. ◎學(xué)術(shù)兼職 中國化學(xué)會生物物理化學(xué)專業(yè)委員會委員(2012.1-)，副主任（2018.07-） 中國生物物理學(xué)會單分子生物學(xué)分會理事(2015.07-) 山東省物理學(xué)會生物物理專業(yè)委員會副主任(2015.12-) ◎指導(dǎo)研究生情況 自2009年以來已指導(dǎo)10余名博士研究生，20多名碩士研究生，其中多人曾獲國家獎學(xué)金，多人畢業(yè)后出國或者在讀期間到國外參加聯(lián)合培養(yǎng)。 ◎榮譽稱號 山東省杰出青年基金獲得者 教育部新世紀(jì)優(yōu)秀人才人選者 | ||||||||||||||||||
